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Objective To evaluate the effects of interleukin-22 (IL-22) on the expression of CC chemokine ligand 27 (CCL27) in human epidermal keratinocytes,and to explore its mechanism.Methods Immunohistochemical study was performed to determine the expression of CCL27 in skin lesions of 10 patients with psoriasis and skin tissues of 5 healthy controls.Cultured HaCaT cells were divided into 8 groups:control group treated with PBS,5 IL-22 groups treated with 12.5,25,50,100 and 200 μg/L IL-22 respectively,2 signaling pathway inhibition groups treated with 50 μrmol/L AG490 (JAK2/STAT3 signaling pathway inhibitor) or PD98059 (MAPK-ERK1/2 signaling pathway inhibitor) for 2 hours followed by the treatment with 50 μg/L IL-22 in the 5％ CO2 incubator at 37 ℃.After 24-hour cultivation,total proteins were extracted,and culture supernatants were collected,and both Western blot analysis and enzyme-linked immunosorbent assay (ELISA) were performed to determine the expression of CCL27.Results Immunohistochemical study showed that the expression of CCL27 was significantly higher in the skin lesions of the patients with psoriasis than in the skin tissues of the healthy controls.Western blot analysis revealed that the protein expression of CCL27 in the 12.5-,25-,50-,100-and 200-μg/L IL-22 groups was 0.491 ± 0.013,0.620 ± 0.019,0.623 ± 0.014,0.802 ± 0.052 and 1.138 ± 0.013 respectively,which were all higher than that in the control group (0.413 ± 0.013,all P ＜ 0.01).The expression of CCL27 was significantly lower in the IL-22 + AG490 group (0.411 ± 0.019) and IL-22 + PD98059 group (0.280 ± 0.012) than in the 50-μg/L IL-22 group (both P ＜ 0.01).ELISA also showed the same trend of changes in the level of CCL27 in the above groups as Western blot showed.Conclusion IL-22 can promote the expression of CCL27 in HaCaT cells,which may be associated with the MAPK-ERK 1/2 and JAK2/STAT3 signaling pathways.
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Objective To investigate the inhibitory effect of IN5 from chlamydiaphage phiCPG1 capsid protein Vp1 on Chlamydia trachomatis (Ct).Methods PCR was used to amplify IN5 gene from Vp1 DNA of phiCPG1, then the recombinant plasmid pET28a/IN5 was constructed.After transformation, the fusion protein IN5 was induced,identified and purified.Ct was incubated with the purified IN5 protein or Vp1 protein.After 48 h of incubation, the inclusion bodies were counted with iodine staining and indirect immunofluorescence.One-way ANOVA was used to compare the difference of inclusion bodies among groups.If the difference among the groups was statistically significant, the Bonferroni method was used to compare any two mean values.Finally, the inhibitory rate of IN5 protein and Vp1 protein to Ct was calculated.Results IN5 protein from chlamydiaphage phiCPG1 capsid protein Vp1 was successfully obtained.At the same concentration of 53μg/mL,the inhibitory rates of Ct growth in IN5 and in Vp1 groups were 52.42% and 78.04%, respectively.Conclusion IN5 protein has inhibitory effect on the growth of Ct,but the inhibitory rate is lower than that of Vp1, which provides a preliminary clue for searching the dominant region of Vp1 protein inhibiting the growth of Ct.
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Objective To develop a father involvement scale and test the reliability and validity.Methods The scale was developed based on previous studies and depth interviews with more than 20 fathers.Totally 1565 families from 3 provinces were recruited.Item analysis was conducted to identify valid items.Then the construct validity of the scale was tested by exploratory and confirmatory factor analysis (EFA & CFA).Finally the reliability and criterion validity of the scale was examined.Results The scale was consisted of 8 factors wihch were involvement attitude,activities and playing,accompany and taking care of children,attentiveness,discipline and school encouragement,economic support,protection,support mother.They explained 59.94％ of the total variance.Besides,the CFA results demonstrated a satisfactory construct validity of the scale (RMSEA =0.05,NFI =0.95,NNFI =0.96,CFI =0.97).The Cronbach(')s α coefficients of the scale was 0.897,and those of its 8 were 0.828,0.788,0.764,0.781,0.671,0.754,0.528 and 0.693 respectively.Conclusion The Father Involvement Scale with 8 factors has a good reliability and validity,demonstrating it a valid tool to measure father involvement in China.
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Objective To explore the current situation of basic psychological needs satisfaction (BPNS) and the relationship between index of well-being and BPNS among young workers in China.Methods The samples consisting of 5972 young workers were selected by stratified and random sampling according to the proportion.The Chinese vision of the Basic Needs Satisfaction in General Scale and the Index of Well-being Scale were used.Results The overall situation of BPNS of young workers (4.36 ± 0.71) was good,satisfied state of relatedness need (SSRN,4.70 ± 1.05) was the highest,satisfied state of autonomy need (SSAN,3.96 ± 1.21) and blocked state of autonomy need (BSAN,4.08 ± 1.17) were lower,blocked state of relatedness need (BSRN,3.20 ± 1.26) was the lowest; education and income had interaction on SSRN (F0.05(6.5948) =4.068,P ＜ 0.01)while there was no difference between gender on all of the dimensions.Pearson correlation analysis showed that all the dimensions of BPNS were significantly related to the index of well-being;and linear regression analysis showed that SSAN,SSRN and BSAN could forecast the index of well-being (adjR2 =0.339),β =0.457,0.186,-0.102 respectively.Conclusion The overall situation of BPNS of young workers is good ; and adjustment of SSAN,SSRN and BSAN could improve the index of well-being of young workers.